What is the procedure for blood collection?

Intended Use

JINGZ Blood Collection Tubes are used to collect, transport, store and process blood for testing serum, plasma or whole blood in the clinical laboratory and are for professional use.

Venipuncture Technique

WEAR GLOVES DURING VENIPUNCTURE AND WHEN HANDLING BLOOD COLLECTION TUBES TO MINIMIZE EXPOSURE HAZARD.

1. Select tube or tubes appropriate for required specimen.

2. Remove the cover over the valve section of the needle.

3. Thread the needle into the holder. Be sure needle is firmly seated to ensure needle does not unthread during use.

4. Apply tourniquet as necessary (max. 1 minute)

5. Prepare venipuncture site with an appropriate antiseptic. DO NOT PALPATE VENIPUNCTURE AREA AFTER CLEANSING.

6. Place patient’s arm in a downward position.

7. Remove needle shield. Perform venipuncture WITH ARM DOWNWARD AND TUBE CAP UPPER-MOST.

8. Push tube into the holder and onto the needle valve puncturing the rubber diaphragm. Centre tubes in holder when penetrating the cap to prevent sidewall penetration and subsequent premature vacuum loss. Hold in place by pressing the tube with the thumb or finger to ensure complete vacuum draw. The fill mark allows for visual control of the correct filling of the tube. A tolerance of +/-10% is allowed.

9. REMOVE TOURNIQUET AS SOON AS BLOOD APPEARS IN TUBE. DO NOT ALLOW CONTENTS OF TUBE TO CONTACT THE CAP OR END OF THE NEEDLE DURING PROCEDURE.

NOTE: Blood may occasionally leak from the needle sleeve. Practice universal standard precautions to minimize hazard exposure. If no blood flows into tube or if blood flow ceases before an adequate specimen is collected, the following steps are suggested to complete satisfactory collection:

a) Ensure the tube is pushed fully forward in the holder.

b) Confirm correct position of needle in vein.

c) If blood still does not flow, remove tube and place new tube onto the holder.

d) If second tube does not draw, remove needle and discard. Repeat procedure from step 1.

10. When the first tube is full and blood flow ceases, gently remove it from holder.

11. Place succeeding tubes in holder, puncturing diaphragm to begin flow. Draw tubes without additives before tubes with additives. Refer to the recommended Order of Draw.

12. Gently invert the tubes immediately after blood collection to reach a proper mix of additive and blood. Turn the filled tube upside-down and return it to upright position. This is one complete inversion.

NOTE: Do not shake the tubes. Vigorous mixing may cause foaming or haemolysis. Insufficient mixing or delayed mixing in serum tubes may result in delayed clotting. In tubes with anticoagulants, inadequate mixing may result in platelet clumping, clotting and /or incorrect test results.

13. As soon as blood stops flowing in the last tube, remove the tube and then the needle from vein, applying pressure to puncture site with dry sterile swab until bleeding stops. Once clotting has occurred, apply bandage if desired.

NOTE: After venipuncture, the top of the cap may contain residual blood. Take proper precautions when handling tubes to avoid contact with this blood. Any needle holder that becomes contaminated with blood is considered hazardous and should be disposed of immediately.

14. Dispose of the used needle with holder using a suitable biohazard disposal container. DO NOT RECAP. Recapping of needles increases the risk of needle stick injury and blood exposure.

15. It is the laboratory’s ultimate responsibility to verify that a change from one tube to another does not significantly affect analytical results obtained from patient samples.

NOTE: Keep the tubes, especially serum, in an upright position.