4ml 5ml Vacuum Blood Test Tube Anticoagulation EDTA Blood Collection Tube

EDTA blood collection tubes (purple cap) utilize dipotassium or tripotassium ethylenediaminetetraacetate (EDTA-K₂ or EDTA-K₃) as the core anticoagulant, featuring:

• Precise Anticoagulation Mechanism: Chelates calcium ions (Ca²⁺) in blood to block thrombin activation, achieving complete anticoagulation while maximally preserving blood cell morphology.
• Optimized Physical Design:
  • High-transparency PET plastic or glass tube with mechanical strength and light resistance
  • Pre-filled precise anticoagulant dosage (1.5-2.2 mg EDTA/mL blood), vacuum accuracy ≤±5%
• Safety & Quality Control:
  • Gamma-irradiated sterilization (8-25 kGy), controlled endotoxin levels
  • ISO13485/CE compliant; standardized purple cap for rapid identification

Primarily for hematological testing, with specific applications:

• Anticoagulant Risks:
  • Overfill (>2.2 mg/mL): RBC shrinkage/platelet swelling -> morphology distortion
  • Underfill: Inadequate anticoagulation -> microclots -> instrument obstruction
• Sample Handling:
  • Do not reuse tubes or mix additives
  • Blood volume ≤ labeled capacity (e.g., ≤5.5 mL for 5 mL tube)
• Safety: EDTA is corrosive; rinse skin/eyes immediately with water if contacted

• EDTA-K₂ vs. EDTA-K₃?
  • Solubility: K₃ dissolves easier -> liquid pre-filled tubes; K₂ -> spray-dried plastic tubes
  • Performance: Comparable anticoagulation, but K₃ excess may cause platelet satellitism (pseudothrombocytopenia)
• Causes of Pseudothrombocytopenia? Solutions?
  • Causes: EDTA-induced platelet aggregation (~0.1% incidence) or neutrophil adhesion (satellitism)
  • Resolution: Recollect using sodium citrate tube
• Can tubes with microclots be used? No. Microclots indicate anticoagulation failure -> recollect with thorough mixing
• Suitable for glucose testing? Compatible with sodium fluoride (2-4 mg/mL blood) to inhibit glycolysis; validate system compatibility